Isolation and Characterization of Buffalo Wharton’s Jelly Derived Mesenchymal Stem Cells
نویسنده
چکیده
Fetal and adult stem cells have the potential to play vital role in livestock production and management. Fetal stem cells in comparison to stem cells from other sources are easily accessible and can be isolated in larger quantities. Additionally it can provide new insights into development biology of animals. Fetal stem cells may develop as a valuable source of stem cells for clinical applications in large animals. According to ISCT (International Society for Cellular Therapy) mesenchymal stem cells can be defined by three criteria 1) Mesenchymal stem cells must be plastic-adherent when maintained in vitro; (2) Mesenchymal stem cells must express CD73, CD90 and CD105, and lack expression of CD34, CD45, CD14 or CD11b, CD79a or CD19 and MHC class II antigens; (3) The MS cells must differentiate into osteoblasts, adipocytes and chondroblasts in vitro [1].
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